Environment friendly Microfluidic-Primarily based Air Sampling/Monitoring Platform for Detection of Aerosol SARS-CoV-2 On-site
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Anal Chem. 2021 Feb 26. doi: 10.1021/acs.analchem.0c05154. On-line forward of print.
Airborne pathogens have been thought of as extremely infectious and transmittable between people. With the pandemic outbreak of the coronavirus illness 2019 (COVID-19), an on-site diagnostic system-integrated airborne pathogen-monitoring machine is advisable by specialists for stopping and controlling the early stage β-coronavirus extreme acute respiratory syndrome coronavirus 2 (SARS-CoV-2) unfold. On this work, a small-volume rotating microfluidic fluorescence chip-integrated aerosol SARS-CoV-2 sampling system was constructed to fulfill the demand for speedy on-site pattern assortment and detection of SARS-CoV-2. The rotating microfluidic fluorescence system with small quantity has very excessive sensitivity within the detection of SARS-CoV-2 (detection restrict of 10 copies/μL with the shortest Ct worth of 15 min), which is similar to reverse transcription polymerase chain response (RT-PCR). The precision variation coefficients inside/between batches are very low [coefficient of variation (CV) % ≤ 5.0%]. Our work has handed the great inspection of the microfluidic chip efficiency by the Shanghai Medical Machine Testing Institute [National Medical Inspection (Design) no. 4408] and efficiently examined 115 medical samples. The built-in system displays 100% specificity, excessive sensitivity (10 copies/μL), and good precision (CV % ≤ 5.0%) within the speedy detection of SARS-CoV-2, thus realizing speedy monitoring and diagnostics of SARS-CoV-2 nucleic acid on-site.